Difference between revisions of "Amitryptyline Treatment of Mice"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (wrote protocol for amitriptyline in water) |
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* Makes 1L of drinking water | * Makes 1L of drinking water | ||
| − | * dissolve 0. | + | * dissolve 0.060g in 1L water |
===Calculation=== | ===Calculation=== | ||
| − | * 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg | + | * 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg drug/kg/mL |
| − | * average mouse weighs 0.03kg so want 0.06 mg | + | * average mouse weighs 0.03kg so want 0.06 mg drug/mL or 60 mg/L |
*This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored. | *This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored. | ||
Latest revision as of 21:21, 28 January 2014
We use Amitiptyline from Sigma (A8404).
For drinking water administration ~10 mg/kg/day
We have approval for 1-100 mg/kg/day. Still need to pilot these effects
- Makes 1L of drinking water
- dissolve 0.060g in 1L water
Calculation
- 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg drug/kg/mL
- average mouse weighs 0.03kg so want 0.06 mg drug/mL or 60 mg/L
- This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored.
