Difference between revisions of "CHAPS Lysis Buffer"
From Bridges Lab Protocols
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see PMID 19200882 | see PMID 19200882 | ||
[[ Category:Buffer ]] | [[ Category:Buffer ]] | ||
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Latest revision as of 18:16, 26 August 2009
CHAPS Lysis Buffer (for 10mL lysis buffer)
| Final Concentration | per 10 mL | Stock | Location | |
|---|---|---|---|---|
| HEPES, pH 7.4 | 40 mM | 400 uL | 1M | 4C |
| NaCl | 120 mM | 300 uL | 4M | Shelf |
| EDTA | 2 mM | 40 uL | 0.5M | Shelf |
| CHAPS | 0.3% | 300 uL | 10% | |
| NaPPi | 10 mM | 400 uL | 0.25M | Shelf |
| beta-glycerophosphate | 10 mM | 100 uL | 1M | 4C |
| NaF | 50 mM | 1mL | 0.5M | Shelf |
| Protease Inhibitors | 1 mini tablet | 4C |
see PMID 19200882
