Difference between revisions of "Tamoxifen Injections for Inducible Knockouts"

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* Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)
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* Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)-- must wear nitrile gloves when dealing with tamoxifen.
 
* Inject 50 mg/kg on five consecutive days, at the same time each day.  The time is important, otherwise they will overdose if you inject too close together.   
 
* Inject 50 mg/kg on five consecutive days, at the same time each day.  The time is important, otherwise they will overdose if you inject too close together.   
 
* To determine how much to inject weigh the mouse and inject 5 uL/g of mouse.  This means a 25 g mouse will get an injection of 125 uL.
 
* To determine how much to inject weigh the mouse and inject 5 uL/g of mouse.  This means a 25 g mouse will get an injection of 125 uL.
 
* Give yourself plenty of time to fill needles because the corn oil takes a long time to aspirate--watch for bubbles.
 
* Give yourself plenty of time to fill needles because the corn oil takes a long time to aspirate--watch for bubbles.
 
* Tamoxifen can be stored at room temperature for up to a week.
 
* Tamoxifen can be stored at room temperature for up to a week.
 +
* The initial weights can be used for the rest of the week when determining injection volume, unless there is reason to believe they will be changing drastically.
  
 
* Notes about transferring mice:  
 
* Notes about transferring mice:  

Revision as of 15:45, 27 April 2017

  • Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)-- must wear nitrile gloves when dealing with tamoxifen.
  • Inject 50 mg/kg on five consecutive days, at the same time each day. The time is important, otherwise they will overdose if you inject too close together.
  • To determine how much to inject weigh the mouse and inject 5 uL/g of mouse. This means a 25 g mouse will get an injection of 125 uL.
  • Give yourself plenty of time to fill needles because the corn oil takes a long time to aspirate--watch for bubbles.
  • Tamoxifen can be stored at room temperature for up to a week.
  • The initial weights can be used for the rest of the week when determining injection volume, unless there is reason to believe they will be changing drastically.
  • Notes about transferring mice:
    • Mice must be transferred to the biocontainment room during injections and for an additional 72 hrs post final injection.
    • The husbandry staff need to know a few days prior to transfer so they can have the room ready for occupancy (i.e. everything stocked and agent filled), for this email sanderjo@med.umich.edu to tell him when and how many cages you you plan to transfer.
    • If you are introducing a new chemical or biological hazard, they need to be made aware of this.
    • There are special barcodes for this room so you must change them out when you transfer in and then again change them to the regular ones when transferring back out.
    • Remember to mark the cage with the appropriate sticker (biological or chemical hazard) and place appropriate acetate with required information (name of agent, dates of administration, and dose)---if you are treating the water with a drug the bottle must be marked was well.
    • Make sure to provide animals with food and water.
    • Additional PPE is required and highly monitored in this area, make sure to abide with these regulations!