Difference between revisions of "YPD Media and Agar"
From Bridges Lab Protocols
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Latest revision as of 16:46, 1 June 2012
Materials
- YPD Medium (Clontech 630409)
- Agar (if making plates)
- Adenine Hemisulfate if making YPDA. Dissolve to 0.2% and autoclave.
Preparation
- Weigh out 5g/100 mL of media
- If making plates, add 2g/100mL of bacto-agar
- Stir to dissolve and check that pH is at 5.8
- Autoclave for 15 min at 121C using the liquid cycle
- If making YPDA plates let cool to ~55C add 1.5 mL/100mL adenine
- If making rapamycin plates let cool to ~55C add desired amount of rapamycin
- Pour plates, let dry and store in the cold room
