Difference between revisions of "Amitryptyline Treatment of Mice"

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(wrote protocol for amitriptyline in water)
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Revision as of 16:04, 23 January 2014


We use Amitiptyline from Sigma (A8404).

For drinking water administration ~10 mg/kg/day

We have approval for 1-100 mg/kg/day. Still need to pilot these effects

  • Makes 1L of drinking water
  • dissolve 0.092g in 1L water

Calculation

  • 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg Dex/kg/mL
  • average mouse weighs 0.03kg so want 0.06 mg Dex/mL or 60 mg/L
  • This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored.