Genotyping Program
From Bridges Lab Protocols
| Allele | Program | Fragment Size(s) | Notes |
|---|---|---|---|
| TSC-floxed | Cre PCR | KO- 368bp, WT- 295bp | Two bands if heterozygote |
| Cre | Cre PCR | Only present if Transgene Positive (cannot detect homozygosity at transgene) | |
| Vac14 | Cre PCR | Two bands if heterozygote | |
| TC10b | TC10b | KO - 500bp, WT-1kb | Two bands if heterozygote |
| ob/ob Genotyping | ob/ob | KO - 123bp, WT-104kb, control 191 bp | Two reactions, see ob/ob Genotyping |
| MTM1 | MTM1 | Mutant-575bp, WT-714bp | Separate Reactions for WT and KO |
| CAP | CAP | Two bands if heterozygote, upper band for KO | |
| Vinexin | Vinexin | ||
| GR KO | nr3c1 | KO- 300bp, WT- 247bp | Two bands if heterozygote |
| mc4r-Cone | mc4r | KO- 540bp, WT- 400bp | Two bands if heterozygote (run primers separately) |
| PTG | ppp1r3c (PTG) | KO- 574bp, WT- 678bp | Two bands if heterozygote (run primers separately) |
| 4EBP1/2 | Cre PCR | 1= KO- 550bp, WT- 175bp; 2= KO- 750bp, WT- 450bp | Two bands if heterozygote for each allele (run primers for 1 and 2 separately) |
| GDF15 | GDF15 | KO- 200bp, WT- 600bp | Two bands if heterozygote (run primers separately) |
