Transfer Buffer
From Bridges Lab Protocols
Revision as of 19:34, 11 July 2013 by Mpeloqu1 (Talk | contribs) (→10X Transfer Buffer: Changed from 10L total to 4L total)
10X Transfer Buffer
To make 1X TBS, dilute 10X with water, and add 20% methanol. For example:
- 100 mL 10X Buffer
- 200 mL Methanol
- 700 mL Water
| Component | Weight per 4 L | Catalog Number |
|---|---|---|
| Tris Base | 121.2 g | Sigma T6066-5KG |
| Glycine | 576.4 g | Sigma G7126-5KG |
