User contributions
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- 19:48, 7 December 2009 (diff | hist) . . (+43) . . m FM4-64 Labeling of Yeast Cells
- 19:40, 7 December 2009 (diff | hist) . . (+56) . . FM4-64 Labeling of Yeast Cells (added SynaptoRed concentration)
- 16:47, 7 December 2009 (diff | hist) . . (+2) . . m FM4-64 Labeling of Yeast Cells
- 16:47, 7 December 2009 (diff | hist) . . (+453) . . FM4-64 Labeling of Yeast Cells (added details from Emily)
- 14:18, 30 November 2009 (diff | hist) . . (+168) . . Lipofectamine Plasmid Transfection
- 15:46, 18 November 2009 (diff | hist) . . (+57) . . m Generating and Displaying an Electostatic Surface (current)
- 15:38, 18 November 2009 (diff | hist) . . (+177) . . Generating and Displaying an Electostatic Surface
- 17:13, 4 November 2009 (diff | hist) . . (+10) . . m Purification of GST-HA-S6K
- 15:32, 4 November 2009 (diff | hist) . . (+82) . . m Purification of GST-HA-S6K
- 15:32, 4 November 2009 (diff | hist) . . (+1,071) . . N Purification of GST-HA-S6K (initial page)
- 14:50, 4 November 2009 (diff | hist) . . (-5) . . HNTG Buffer (current)
- 14:49, 4 November 2009 (diff | hist) . . (+368) . . N HNTG Buffer (Created page with ' Category:Buffer Category:Immunoprecipitation {| border="1" ! || Final Concentration || per 10 mL || Stock || Location |- | HEPES, pH 7.4 || 50 mM || 500 uL || 1M || 4C...')
- 21:24, 3 November 2009 (diff | hist) . . (-75) . . PCR Amplification of DNA (KOD Polymerase) (current)
- 21:24, 3 November 2009 (diff | hist) . . (-14) . . m PCR Amplification of DNA (KOD Polymerase)
- 21:23, 3 November 2009 (diff | hist) . . (+1,258) . . Nm PCR Amplification of DNA (KOD Polymerase) (initial page)
- 16:27, 27 October 2009 (diff | hist) . . (+102) . . m Preparation of DIG Labelled Probes (current)
- 16:27, 27 October 2009 (diff | hist) . . (+40) . . m Main Page (→Protein Analysis)
- 16:26, 27 October 2009 (diff | hist) . . (+921) . . N Preparation of DIG Labelled Probes (wrote initial page)
- 15:43, 21 October 2009 (diff | hist) . . (+82) . . m Colony PCR (current)
- 15:43, 21 October 2009 (diff | hist) . . (+17) . . m Main Page (→Genotyping)
- 15:42, 21 October 2009 (diff | hist) . . (+608) . . N Colony PCR (Created page with '==Reagents== *Primers *dNTPs (2 mM of each dNTP) *Expand polymerase (Roche) *Primers *Yeast Colonies ==Protocol== #Pick about 20% of a colony and mix in 20 uL of sterile water. ...')
- 14:50, 20 October 2009 (diff | hist) . . (+2) . . Differentiation of 3T3-L1 Cells
- 18:30, 14 October 2009 (diff | hist) . . (+11) . . m Glucose Uptake Assay
- 18:27, 14 October 2009 (diff | hist) . . (+455) . . N KRBH Buffer (initial page) (current)
- 18:21, 14 October 2009 (diff | hist) . . (+277) . . Rapamycin Sensitivity Assay of Yeast (added notes about dilutions and incubations) (current)
- 18:19, 14 October 2009 (diff | hist) . . (+11) . . Glucose Uptake Assay (added link to KRBH Buffer)
- 15:05, 13 October 2009 (diff | hist) . . (+57) . . m Rapamycin Sensitivity Assay of Yeast
- 13:28, 13 October 2009 (diff | hist) . . (+578) . . N Rapamycin Sensitivity Assay of Yeast (Created page with '==Materials== *YPD Media *YPD Plates with either DMSO or 2-200 nM Rapamycin *Yeast strains, streaked out on appropriate plate (< 1 month old) ==Protocol== #Innoculate 3 mL of YP...')
- 00:53, 10 October 2009 (diff | hist) . . (+2) . . m Generating and Displaying an Electostatic Surface
- 00:52, 10 October 2009 (diff | hist) . . (+6) . . m Generating and Displaying an Electostatic Surface
- 00:52, 10 October 2009 (diff | hist) . . (+376) . . m Generating and Displaying an Electostatic Surface
- 00:39, 10 October 2009 (diff | hist) . . (+76) . . m Generating and Displaying an Electostatic Surface
- 00:38, 10 October 2009 (diff | hist) . . (+72) . . m Generating and Displaying an Electostatic Surface (→Generate PQR File for the Structure)
- 00:20, 10 October 2009 (diff | hist) . . (+260) . . N Generating and Displaying an Electostatic Surface (Created page with '==Programs Required== *Pymol *APBS ===Generate PQR File for the Structure=== #Download or generate PDB file. #Go to the PDB2PQR server at http://pdb2pqr-1.wustl.edu/pdb2pqr/. T...')
- 12:57, 9 October 2009 (diff | hist) . . (+41) . . YPD Media and Agar
- 12:57, 9 October 2009 (diff | hist) . . (+521) . . N YPD Media and Agar (Created page with '==Materials== *YPD Medium (Clontech 630409) *Agar (if making plates) *Adenine Hemisulfate if making YPDA. Dissolve to 0.2% and autoclave. ==Preparation== *Weigh out 5g/100 mL o...')
- 19:57, 5 October 2009 (diff | hist) . . (+276) . . N FM4-64 Labeling of Yeast Cells (initial page)
- 14:31, 1 October 2009 (diff | hist) . . (+41) . . m Designing and Preparing dsRNA (current)
- 14:31, 1 October 2009 (diff | hist) . . (+1,236) . . N Using ImageJ to Quantify Bands (updated and clarified some points) (current)
- 20:14, 29 September 2009 (diff | hist) . . (-26) . . m DsRNA Mediated Knockdown of S2 Cells (current)
- 20:13, 29 September 2009 (diff | hist) . . (+23) . . m DsRNA Mediated Knockdown of S2 Cells
- 20:11, 29 September 2009 (diff | hist) . . (-113) . . m DsRNA Mediated Knockdown of S2 Cells
- 20:11, 29 September 2009 (diff | hist) . . (+113) . . m DsRNA Mediated Knockdown of S2 Cells
- 20:07, 29 September 2009 (diff | hist) . . (+46) . . m DsRNA Mediated Knockdown of S2 Cells
- 20:07, 29 September 2009 (diff | hist) . . (+17) . . m DsRNA Mediated Knockdown of S2 Cells
- 20:05, 29 September 2009 (diff | hist) . . (+190) . . m DsRNA Mediated Knockdown of S2 Cells
- 20:03, 29 September 2009 (diff | hist) . . (-20) . . m DsRNA Mediated Knockdown of S2 Cells
- 20:00, 29 September 2009 (diff | hist) . . (+809) . . N DsRNA Mediated Knockdown of S2 Cells (initial page)
- 15:40, 16 September 2009 (diff | hist) . . (+50) . . m Designing and Preparing dsRNA (→Preparing dsRNA)
- 15:38, 16 September 2009 (diff | hist) . . (+5) . . m Designing and Preparing dsRNA
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