User contributions
(newest | oldest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 14:57, 2 December 2013 (diff | hist) . . (+1,583) . . N Quantifying Relative Expression from qRT-PCR (Wrote initial page) (current)
- 13:52, 31 October 2013 (diff | hist) . . (+14) . . Primer Design for qPCR (added direction to primer name)
- 13:38, 31 October 2013 (diff | hist) . . (+2,251) . . N QPCR (Wrote page for qPCR)
- 13:10, 15 October 2013 (diff | hist) . . (+26) . . Parsing XSQ Files (added filenames to command) (current)
- 21:32, 14 October 2013 (diff | hist) . . (+1,095) . . N Primer Design for qPCR (wrote protocol)
- 21:15, 14 October 2013 (diff | hist) . . (+644) . . N Parsing XSQ Files (copied out instructions.)
- 17:17, 7 October 2013 (diff | hist) . . (+216) . . N Creating New R Packages (wrote new page) (current)
- 19:59, 26 September 2013 (diff | hist) . . (+482) . . Ob/ob Genotyping (added nots on how to make primer mixes and added band sizes) (current)
- 14:17, 23 September 2013 (diff | hist) . . (+13) . . Differentiation of 3T3-L1 Cells (updated MIX stock solution instructions)
- 15:07, 18 September 2013 (diff | hist) . . (+82) . . m Rapamycin Stock Solution (added categories) (current)
- 15:06, 18 September 2013 (diff | hist) . . (+285) . . N Rapamycin Stock Solution (wrote initial page)
- 15:55, 12 September 2013 (diff | hist) . . (+436) . . N Culturing MIN6 Cells (wrote initial page) (current)
- 15:04, 12 September 2013 (diff | hist) . . (+485) . . N Measuring Autophagy in Tissue Culture Cells (added notes about bafilomycin treatment) (current)
- 13:52, 27 August 2013 (diff | hist) . . (+135) . . High Fat Diet Treatment (added note about ordering C57BL/6J mice)
- 00:24, 16 July 2013 (diff | hist) . . (-1) . . Bridges Lab Protocols:About
- 16:11, 15 July 2013 (diff | hist) . . (+338) . . Preparation of Protein Lysates from Mouse Tissues (updated protocol with new weights)
- 14:37, 9 July 2013 (diff | hist) . . (+120) . . m Cre PCR (added categories)
- 14:36, 9 July 2013 (diff | hist) . . (-2) . . m Cre PCR
- 14:35, 9 July 2013 (diff | hist) . . (+96) . . N Cre PCR (copied in PCR program)
- 14:34, 9 July 2013 (diff | hist) . . (+7) . . Genotyping Program (added link to Cre PCR program)
- 14:55, 5 July 2013 (diff | hist) . . (+495) . . N TC10b Genotyping (wrote initial protocol) (current)
- 15:53, 19 June 2013 (diff | hist) . . (+131) . . N Bridges Lab Protocols:Copyrights (added link to CC0 for copyrights page) (current)
- 15:42, 19 June 2013 (diff | hist) . . (+546) . . Main Page (Wrote greeting and reformatted main page)
- 15:07, 12 April 2013 (diff | hist) . . (+90) . . Western Blotting (added links to buffers)
- 15:05, 12 April 2013 (diff | hist) . . (+366) . . N SDS-PAGE Running Buffer (copied recipe from saltiel lab buffers) (current)
- 14:57, 12 April 2013 (diff | hist) . . (+357) . . N Transfer Buffer (copied recipe from saltiel lab buffers)
- 14:52, 12 April 2013 (diff | hist) . . (+344) . . N TBST (copied recipe from saltiel lab buffers) (current)
- 16:57, 22 February 2013 (diff | hist) . . (+34) . . m Lipofectamine Transfection of C2C12 Cells (changed confluence note) (current)
- 16:56, 22 February 2013 (diff | hist) . . (+1,325) . . N Lipofectamine Transfection of C2C12 Cells (Wrote protocol from ethan's trials)
- 16:45, 22 February 2013 (diff | hist) . . (+446) . . N Talk:Fugene Transfection of C2C12 Cells (ethan provided his protocol) (current)
- 20:42, 18 February 2013 (diff | hist) . . (+28) . . Glucose Uptake Assay (specified insulin concentration)
- 15:20, 21 January 2013 (diff | hist) . . (+96) . . Genotyping Program (added MTM1 program)
- 15:17, 21 January 2013 (diff | hist) . . (+120) . . m MTM1 Genotyping (Added categories) (current)
- 15:16, 21 January 2013 (diff | hist) . . (+42) . . MTM1 Genotyping (added sample pcr result)
- 15:15, 21 January 2013 (diff | hist) . . (+158) . . N File:MTM1 Genotyping Result.jpg (Expected results of Genotyping program, from http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153) (current)
- 15:13, 21 January 2013 (diff | hist) . . (+501) . . N MTM1 Genotyping (wrote protocol for MTM1 genotyping)
- 14:58, 11 January 2013 (diff | hist) . . (+66) . . Maintenance of Fly Stocks (added food cost) (current)
- 19:11, 30 December 2012 (diff | hist) . . (+142) . . m TSC-floxed Genotyping (added categories) (current)
- 19:10, 30 December 2012 (diff | hist) . . (+891) . . N TSC-floxed Genotyping (Added primers and reference for TSC1 primers)
- 18:38, 14 December 2012 (diff | hist) . . (+66) . . N Talk:Triglyceride Assay from Cells and Tissues (added alternative protocol from ron kahn) (current)
- 21:04, 4 December 2012 (diff | hist) . . (0) . . 5' RACE (→PRC of dC-Tailed cDNA) (current)
- 21:04, 4 December 2012 (diff | hist) . . (+822) . . 5' RACE (Added PCR reaction)
- 14:49, 4 December 2012 (diff | hist) . . (+1,048) . . 5' RACE (Added purification and tailing protocols)
- 14:36, 4 December 2012 (diff | hist) . . (+1,599) . . N 5' RACE (Rewrote protcol for first strand synthesis)
- 14:51, 30 November 2012 (diff | hist) . . (+1,499) . . N Measuring Lifespan in Drosophila (wrote initial protocol based on Broughton et al) (current)
- 14:23, 30 November 2012 (diff | hist) . . (+11) . . m Talk:Measuring Lifespan in Drosophila (updated link to PLOS paper) (current)
- 18:10, 29 November 2012 (diff | hist) . . (+920) . . Talk:Measuring Lifespan in Drosophila (added ason's papers)
- 17:47, 25 November 2012 (diff | hist) . . (+768) . . N Talk:Measuring Lifespan in Drosophila (added papers suggested by matt piper)
- 19:29, 21 November 2012 (diff | hist) . . (+112) . . Maintenance of Fly Stocks (added note about expanding colonies)
- 15:08, 20 November 2012 (diff | hist) . . (+1) . . m High Fat Diet Treatment (error in sucrose amount)
(newest | oldest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
