User contributions
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- 20:02, 13 July 2015 (diff | hist) . . (+112) . . Surveyor Assay for Genomic DNA Mutations (added categories) (current)
- 13:56, 24 June 2015 (diff | hist) . . (0) . . m Surveyor Assay for Genomic DNA Mutations (changed volume of polymerase)
- 18:01, 26 May 2015 (diff | hist) . . (+1,631) . . N Surveyor Assay for Genomic DNA Mutations (wrote initial protocol)
- 15:30, 20 April 2015 (diff | hist) . . (-1) . . m Oil red o stain for in vitro adipogenesis (fixed isoproterenol format error) (current)
- 15:29, 20 April 2015 (diff | hist) . . (-48) . . m Oil red o stain for in vitro adipogenesis (removed redundant title)
- 15:29, 20 April 2015 (diff | hist) . . (+50) . . m Oil red o stain for in vitro adipogenesis (updated format)
- 14:44, 19 February 2015 (diff | hist) . . (+4) . . m Paraffin Embedding of Tissue Samples
- 14:43, 19 February 2015 (diff | hist) . . (+753) . . Paraffin Embedding of Tissue Samples (split table to allow for entering times)
- 18:52, 30 December 2014 (diff | hist) . . (+1,491) . . N TaqMan miRNA Assay (wrote first half) (current)
- 21:35, 29 December 2014 (diff | hist) . . (+1,794) . . N Purification of miRNA and mRNA with TRIzol (wrote initial page) (current)
- 21:44, 1 December 2014 (diff | hist) . . (+486) . . N Tamoxifen Injections for Inducible Knockouts (wrote initial page)
- 14:57, 11 November 2014 (diff | hist) . . (+119) . . Primary Adipocyte Isolation (added categories and RPM) (current)
- 14:51, 11 November 2014 (diff | hist) . . (+1,685) . . N Lipolysis Assay from Primary Adipocytes (wrote initial page) (current)
- 14:25, 11 November 2014 (diff | hist) . . (+1,351) . . N Primary Adipocyte Isolation (wrote initial page)
- 14:16, 29 October 2014 (diff | hist) . . (+12) . . Designing and Generating CRISPR-Cas Mutants (updated with links to CRISPR design tool)
- 13:59, 29 October 2014 (diff | hist) . . (+219) . . Designing and Generating CRISPR-Cas Mutants (updated with pX459)
- 22:12, 17 September 2014 (diff | hist) . . (+78) . . QPCR (added booking site)
- 14:51, 31 July 2014 (diff | hist) . . (+125) . . Primer Design for qPCR (updated specifying to start with the ncbi gene page)
- 13:12, 11 July 2014 (diff | hist) . . (+148) . . N Actinomycin D (wrote initial page) (current)
- 12:55, 11 July 2014 (diff | hist) . . (+156) . . N Cycloheximide (created page) (current)
- 12:50, 24 June 2014 (diff | hist) . . (+20) . . Glycogen Determination from Tissues (updated product number for amyloglucosidase)
- 12:33, 29 April 2014 (diff | hist) . . (+1,961) . . N Triglyceride Assay from Tissue Culture Cells (wrote protocol for cells)
- 12:30, 29 April 2014 (diff | hist) . . (+47) . . Triglyceride Assay from Cells and Tissues (Undo revision 853 by Davebridges (talk))
- 12:30, 29 April 2014 (diff | hist) . . (-47) . . Triglyceride Assay from Cells and Tissues (Updated protocol for tissue culture)
- 10:43, 10 April 2014 (diff | hist) . . (0) . . High Fat Diet Treatment (updated caloric content of HFD)
- 14:38, 9 March 2014 (diff | hist) . . (+618) . . Designing and Generating CRISPR-Cas Mutants (added notes about verifying clones)
- 15:53, 6 March 2014 (diff | hist) . . (+262) . . Designing and Generating CRISPR-Cas Mutants (added note about naming of gDNA sequences)
- 15:14, 6 March 2014 (diff | hist) . . (+307) . . Designing and Generating CRISPR-Cas Mutants (→Designing the Targeting Strategy: added note about avoiding spanning an intron)
- 18:34, 5 March 2014 (diff | hist) . . (+646) . . N Sending Samples for DNA Sequencing (Created page with "# Create an account or log in at http://128.169.4.12/ # Select Economy under Service Requested and enter the number of samples in the box above. # Select '''Enter Individual ...") (current)
- 16:22, 1 March 2014 (diff | hist) . . (+68) . . N Category:Cloning (Created page with "This includes protocols for cloning and working with recombinant DNA") (current)
- 16:22, 1 March 2014 (diff | hist) . . (+49) . . m Main Page (→Cloning and Molecular Biology: added crispr cloning to index page)
- 16:19, 1 March 2014 (diff | hist) . . (+254) . . N Talk:Designing and Generating CRISPR-Cas Mutants (added notes about diluting insert) (current)
- 16:17, 1 March 2014 (diff | hist) . . (+205) . . Designing and Generating CRISPR-Cas Mutants (updated protocol after successful cloning)
- 21:02, 26 February 2014 (diff | hist) . . (+127) . . Designing and Generating CRISPR-Cas Mutants (added categories)
- 21:00, 26 February 2014 (diff | hist) . . (+59) . . Designing and Generating CRISPR-Cas Mutants (updated annealing conditions)
- 20:28, 26 February 2014 (diff | hist) . . (+840) . . Designing and Generating CRISPR-Cas Mutants (Added cloning information)
- 18:01, 26 February 2014 (diff | hist) . . (+8) . . Designing and Generating CRISPR-Cas Mutants (updated image schematic)
- 17:54, 26 February 2014 (diff | hist) . . (+95) . . N File:PX335.png (Image of pX335 multiple cloning site, taken from the Addgene site http://www.addgene.org/42335/) (current)
- 17:53, 26 February 2014 (diff | hist) . . (+1,856) . . N Designing and Generating CRISPR-Cas Mutants (added initial page)
- 17:20, 14 February 2014 (diff | hist) . . (+37) . . Measuring Grip Strength (added blanking step) (current)
- 17:20, 14 February 2014 (diff | hist) . . (+1,135) . . N Measuring Grip Strength (wrote grip protocol)
- 16:07, 12 February 2014 (diff | hist) . . (+920) . . N Making LB Agar Plates (wrote page for making plates)
- 15:44, 12 February 2014 (diff | hist) . . (-115) . . Transformation of Bacteria (updated transformation protocol) (current)
- 16:58, 10 February 2014 (diff | hist) . . (+4) . . Splitting Cells (added temperature)
- 21:21, 28 January 2014 (diff | hist) . . (0) . . Amitryptyline Treatment of Mice (current)
- 21:19, 28 January 2014 (diff | hist) . . (+2) . . m Amitryptyline Treatment of Mice (fixed two typos)
- 16:04, 23 January 2014 (diff | hist) . . (+668) . . N Amitryptyline Treatment of Mice (wrote protocol for amitriptyline in water)
- 16:39, 17 January 2014 (diff | hist) . . (0) . . m Insulin Tolerance Test (fixed glucose typo)
- 15:29, 17 January 2014 (diff | hist) . . (+61) . . Insulin Tolerance Test (added ordering details for humulin)
- 14:22, 5 December 2013 (diff | hist) . . (+491) . . Using Github and Git for Code (updated with instructions about pulling in from remote repositories.) (current)
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