Nr3c1 Genotyping

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PRIMERS

nr3c1

Fwd: AAACAGGGTTATGCTTGGCA
Rev: TGCCTGCTAGGCAAATGATCT

Cre

Fwd: GCATT ACCGG GCAAC GAGTG ATGAG
Rev: GAGTG AACGA ACCTG GTCGA AATCA GTGCG

Make nr3c1 primer dilution at 1uM (10uL each primer + 980uL water) and Cre at .4uM. Primers in Genotyping Box are marked with a star.

PCR PROTOCOL

94 °C 2 min
10 cycles of:
- 94 °C 20 s
- 65 °C 15 s
- 68 °C 10 s
28 cycles of:
- 94 °C 15 s
- 60 °C 15 s
- 72 °C 10 s
72 °C 2 min

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