BDNF PCR

From Bridges Lab Protocols
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BDNF Primers

  • oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common)
  • oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse)
  • oIMR01694 --- GGG AAC TTC CTG ACT AGG GG (Reverse)

BDNF Cycler Program

  1. 94C - 10 minutes (HotStart Taq)
  2. 94C - 30 seconds
  3. 55C - 30 seconds
  4. 72C - 30 seconds ----- Repeat steps 2-4 for 35 cycles
  5. 72C - 2 minutes
  6. 4C - Hold

http://jaxmice.jax.org/protocolsdb/f?p=116:2:3011685288607993::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:339,002266

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