Difference between revisions of "Main Page"
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==Media and Buffers== | ==Media and Buffers== | ||
===Yeast Media=== | ===Yeast Media=== | ||
| − | [[YPD Media and Agar]] | + | *[[YPD Media and Agar]] |
| − | [[Yeast Selective Media and Agar]] | + | *[[Yeast Selective Media and Agar]] |
===Buffers=== | ===Buffers=== | ||
====Lysis Buffers==== | ====Lysis Buffers==== | ||
| − | [[KRBH Buffer]] | + | *[[KRBH Buffer]] |
| − | [[RIPA Buffer]] | + | *[[RIPA Buffer]] |
| − | [[CHAPS Lysis Buffer]] | + | *[[CHAPS Lysis Buffer]] |
====SDS-PAGE Buffers==== | ====SDS-PAGE Buffers==== | ||
| − | [[SDS-PAGE Separating Gel Buffer]] | + | *[[SDS-PAGE Separating Gel Buffer]] |
| − | [[SDS-PAGE Stacking Gel Buffer]] | + | *[[SDS-PAGE Stacking Gel Buffer]] |
| − | [[TORC1 Kinase Buffer]] | + | *[[TORC1 Kinase Buffer]] |
| − | [[Acrylamide Solution]] | + | *[[Acrylamide Solution]] |
====Other Buffers==== | ====Other Buffers==== | ||
| − | [[KRBH Buffer]] | + | *[[KRBH Buffer]] |
| − | [[MTORC1 Kinase Assay]] | + | *[[MTORC1 Kinase Assay]] |
| − | [[TORC1 Kinase Buffer]] | + | *[[TORC1 Kinase Buffer]] |
==Figure Generation and Data Management== | ==Figure Generation and Data Management== | ||
*[[Generation of Figures in Illustrator]] | *[[Generation of Figures in Illustrator]] | ||
*[[Using Bioconductor To Analyse Microarray Data]] | *[[Using Bioconductor To Analyse Microarray Data]] | ||
Revision as of 14:10, 15 December 2009
Contents
Cell and Tissue Culture
- Splitting Cells
- Generating DMSO Stocks for Cell Culture
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Fugene Transfection of 293T/COS Cells
- Lipofectamine Mediated Knockdown
- Lipofectamine Plasmid Transfection
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
- Luciferase Assay
- Inositol Labeling and Lipid Extraction
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
- Cesium Chloride Preparation of DNA
Cell Biology
Protein Analysis
Protein Quantification
Protein Purification
- French Press
- Purification of GST Fusion Proteins
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
- Preparation of DIG Labelled Probes
Transcriptional Analysis
- Real Time PCR From Cell Culture
- Harvesting RNA from Cells grown in monolayer
- Using Bioconductor To Analyse Beadarray Data
- Using Bioconductor To Analyse Microarray Data
Mouse Protocols
Genotyping
Metabolic Measurements
Tissue Preparation
- Harvesting Mouse Tissue
- Preparation of Protein Lysates from Mouse Tissues
- Preparation of RNA Samples from Mouse Tissues
