Difference between revisions of "Main Page"
From Bridges Lab Protocols
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*[[Quantification by Absorbance at 280nm]] | *[[Quantification by Absorbance at 280nm]] | ||
*[[Determining Percent Purity]] | *[[Determining Percent Purity]] | ||
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===Protein Purification=== | ===Protein Purification=== | ||
Revision as of 21:17, 17 November 2010
Contents
Cell and Tissue Culture
- Splitting Cells
- Generating DMSO Stocks for Cell Culture
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Fugene Transfection of 293T/COS Cells
- Lipofectamine Mediated Knockdown
- Lipofectamine Plasmid Transfection
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
- Luciferase Assay
- Inositol Labeling and Lipid Extraction
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
- Cesium Chloride Preparation of DNA
Cell Biology
Protein Analysis
Protein Quantification
- Bradford Assay
- Quantification by Absorbance at 280nm
- Determining Percent Purity
- Using ImageJ to Quantify Bands
Protein Purification
- French Press
- Purification of GST Fusion Proteins
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
- Preparation of DIG Labelled Probes
- Glycogen Synthase Assay
- Triglyceride Assay from Cells and Tissues
Transcriptional Analysis
- Real Time PCR From Cell Culture
- Harvesting RNA from Cells grown in monolayer
- Using Bioconductor To Analyse Beadarray Data
- Using Bioconductor To Analyse Microarray Data
Mouse Protocols
Genotyping
Metabolic Measurements
Tissue Preparation
- Harvesting Mouse Tissue
- Preparation of Protein Lysates from Mouse Tissues
- Preparation of RNA Samples from Mouse Tissues
