Difference between revisions of "Main Page"
From Bridges Lab Protocols
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*[[Preparation of Protein Lysates from Mouse Tissues]] | *[[Preparation of Protein Lysates from Mouse Tissues]] | ||
*[[Preparation of RNA Samples from Mouse Tissues]] | *[[Preparation of RNA Samples from Mouse Tissues]] | ||
| + | |||
| + | ==Media and Buffers== | ||
| + | ===Yeast Media=== | ||
| + | [[YPD Media and Agar]] | ||
| + | [[Yeast Selective Media and Agar]] | ||
| + | ===Buffers=== | ||
| + | ====Lysis Buffers==== | ||
| + | [[KRBH Buffer]] | ||
| + | [[RIPA Buffer]] | ||
| + | [[CHAPS Lysis Buffer]] | ||
| + | ====SDS-PAGE Buffers==== | ||
| + | [[SDS-PAGE Separating Gel Buffer]] | ||
| + | [[SDS-PAGE Stacking Gel Buffer]] | ||
| + | [[TORC1 Kinase Buffer]] | ||
| + | [[Acrylamide Solution]] | ||
| + | ====Other Buffers==== | ||
| + | [[KRBH Buffer]] | ||
| + | [[MTORC1 Kinase Assay]] | ||
| + | [[TORC1 Kinase Buffer]] | ||
==Figure Generation and Data Management== | ==Figure Generation and Data Management== | ||
*[[Generation of Figures in Illustrator]] | *[[Generation of Figures in Illustrator]] | ||
*[[Using Bioconductor To Analyse Microarray Data]] | *[[Using Bioconductor To Analyse Microarray Data]] | ||
Revision as of 14:09, 15 December 2009
Contents
Cell and Tissue Culture
- Splitting Cells
- Generating DMSO Stocks for Cell Culture
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Fugene Transfection of 293T/COS Cells
- Lipofectamine Mediated Knockdown
- Lipofectamine Plasmid Transfection
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
- Luciferase Assay
- Inositol Labeling and Lipid Extraction
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
- Cesium Chloride Preparation of DNA
Cell Biology
Protein Analysis
Protein Quantification
Protein Purification
- French Press
- Purification of GST Fusion Proteins
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
- Preparation of DIG Labelled Probes
Transcriptional Analysis
- Real Time PCR From Cell Culture
- Harvesting RNA from Cells grown in monolayer
- Using Bioconductor To Analyse Beadarray Data
- Using Bioconductor To Analyse Microarray Data
Mouse Protocols
Genotyping
Metabolic Measurements
Tissue Preparation
- Harvesting Mouse Tissue
- Preparation of Protein Lysates from Mouse Tissues
- Preparation of RNA Samples from Mouse Tissues
Media and Buffers
Yeast Media
YPD Media and Agar Yeast Selective Media and Agar
Buffers
Lysis Buffers
KRBH Buffer RIPA Buffer CHAPS Lysis Buffer
SDS-PAGE Buffers
SDS-PAGE Separating Gel Buffer SDS-PAGE Stacking Gel Buffer TORC1 Kinase Buffer Acrylamide Solution
Other Buffers
KRBH Buffer MTORC1 Kinase Assay TORC1 Kinase Buffer
