High Salt Immune Complex Wash Buffer

From Bridges Lab Protocols
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Chemical Final Concentration Per 15mL Stock Location
SDS 0.1% 150uL 10% Solutions Shelf
Triton X-100 1% 1.5mL 10% Solutions Shelf (borrowed from Seo Lab to make stock)
EDTA 2mM 60uL 500mM Solutions Shelf
Tris 20mM 300uL 1M Solutions Shelf
NaCl 500mM 1.875mL 4M Solutions Shelf
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